課程將以小鼠為基本動物模型，提供學生進階之哺乳動物胚之生技相關實務操作訓練，課程內容將涵蓋：卵與胚的收集與操作、卵與胚的玻璃化冷凍保存技術、體外受精、胚培養系統評估、卵與胚之精密顯微操作(如：單ㄧ精子顯微注射、體細胞核移置、胚活體檢視、?囊胚注射…等)、全能胚幹細胞系之建立及其體外與體內分析之鑑定等相關技術，提供一系列知識與技術之訓練。
This course will focus on mouse animal model to provide students a state-of-the- art of the technologies in embryo development and pluripotent stem cells (i.e. embryonic stem cells) research and its applications. The course will provide valuable opportunities for students to participate various practical training and knowledge in oocyte and embryo handling, in vitro fertilization, cryopreservation of egg and embryo, micromanipulation techniques (i.e. intracytoplasmic sperm injection and somatic cell nuclear transfer, embryo biopsy, blastocyst injection…etc), derivation and in vitro/in vivo characterizations of pluripotent stem cells.